ERBB2 多抗

品牌: 美国 NewEast Biosciences
货号: 21200
抗原: Synthetic C-terminal peptide
规格: 100 微升
产品全名: Anti-ERBB2 Rabbit polyclonal Antibody
应用: ELISA, WB, IHC
种属反应性的选择: Human, Mouse and Rat
物种/宿主: Rabbit
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             产品描述             

ERBB2 多抗

货号:   21200
基因符号:   ERBB2/HER2/Neu/c-Neu
描述:   Anti-ERBB2 Rabbit polyclonal Antibody
背景介绍:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
免疫原:   A synthetic peptide from the C-terminal region of ERBB2, human origin
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IHC: 1: 50-1: 100
浓度:   0.1 mg/ml
宿主:   Rabbit
形式:   Liquid
克隆性:   Polyclonal
纯度:   Purified from serum
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes Erb-B2 of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing.
Immunohistochemistry:   
image.png
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with anti-Erb-B2 polyclonal antibody (Cat. #21200). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
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