NOTCH1(M2011R) 小鼠单抗

品牌: 美国 NewEast Biosciences
货号: 26444
抗原: Synthetic peptide
规格: 100 微升
产品全名: Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody
应用: WB, IP, IHC
种属反应性的选择: Human, Mouse and Rat
物种/宿主: Mouse
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¥4,800.00

             产品描述             

NOTCH1(M2011R)

货号:   26444
规格:   100 µL
基因符号:   NOTCH1, AOS5, AOVD1,TAN1, hN1
描述:   Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody
背景介绍:   NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
免疫原:   A synthetic peptide from the internal region of NOTCH1, which includes the mutation of M2011R, human origin
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1: 1000-1: 2000
WB: 1: 500-1: 1000
IF: 1: 50-1: 100
IHC: 1: 50-1: 100
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   
Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
免疫荧光:   
Immunofluorescence of cells expressing NOTCH1 proteins with Anti-NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP-NOTCH1 (WT) plasmid (left column) or pCDNA3-GFP-NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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