SMO(A68V) 小鼠单抗

品牌: 美国 NewEast Biosciences
货号: 26425
抗原: Synthetic peptide
规格: 100 微升
产品全名: Anti-SMO(A68V) Mouse Monoclonal Antibody
种属反应性的选择: Human, Mouse and Rat
物种/宿主: Mouse





货号:   26425
规格:   100 µL
基因符号:   SMO; MRD21
描述:   Anti-SMO(A68V) Mouse Monoclonal Antibody
背景介绍:   Smoothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
免疫原:   A synthetic peptide from the internal region of SMO, which includes the mutation of A68V, human origin
经过测试的应用:   ELISA, WB, IHC
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 1000
IHC: 1: 50-1: 100
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes A68V mutant, but not wild-type SMO of vertebrates
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
Western blot analysis of recombinant SMO(A68V) and wild-type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti-SMO(A68V) monoclonal antibody (Cat. #26425).
Immunofluorescence of cells expressing SMO proteins with Anti-SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMO (WT) plasmid (left column) or pCDNA3-GFP-SMO(A68V) plasmid (right column), then fixed and stained with Anti-SMO(A68V) monoclonal antibody (Cat. #26425).



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